Nature Communications (Nov 2016)

Cl-out is a novel cooperative optogenetic tool for extruding chloride from neurons

  • Hannah Alfonsa,
  • Jeremy H. Lakey,
  • Robert N. Lightowlers,
  • Andrew J. Trevelyan

DOI
https://doi.org/10.1038/ncomms13495
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 9

Abstract

Read online

Chloride regulation is important for setting GABAergic reversal potential, though tools to manipulate chloride levels are limited. Here, the authors combine Archaerhodopsin with a chloride channel opsin to generate an optogenetic chloride extrusion strategy, ‘Cl-out’, which they demonstrate in hippocampal slices.