Automated analysis of mitochondrial dimensions in mesenchymal stem cells: Current methods and future perspectives
Sabrina Summer,
Agnes Kocsis,
Eva Ingeborg Reihs,
Mario Rothbauer,
Kirill Lonhus,
Dalibor Stys,
Peter Ertl,
Michael B. Fischer
Affiliations
Sabrina Summer
Department for Biomedical Research, Center of Experimental Medicine, Danube University Krems, Dr.-Karl-Dorrek-Straße 30, Krems an der Donau, Austria; Corresponding author. Danube University Krems Department for Biomedical Research, Center of Experimental Medicine Dr.-Karl-Dorrek-Straße 30, Krems an der Donau 3500, Austria.
Agnes Kocsis
Department for Biomedical Research, Center of Experimental Medicine, Danube University Krems, Dr.-Karl-Dorrek-Straße 30, Krems an der Donau, Austria
Eva Ingeborg Reihs
Karl Chiari Lab for Orthopaedic Biology & Ludwig Boltzmann Institute for Arthritis and Rehabilitation, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, Austria; Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Technical University Vienna, Faculty of Technical Chemistry, Getreidemarkt 9/163, Vienna, Austria
Mario Rothbauer
Karl Chiari Lab for Orthopaedic Biology & Ludwig Boltzmann Institute for Arthritis and Rehabilitation, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, Austria; Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Technical University Vienna, Faculty of Technical Chemistry, Getreidemarkt 9/163, Vienna, Austria
Kirill Lonhus
Institute of Complex Systems, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in České Budějovice, Zámek 136, 373 33 Nové Hrady, Czech Republic
Dalibor Stys
Institute of Complex Systems, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in České Budějovice, Zámek 136, 373 33 Nové Hrady, Czech Republic
Peter Ertl
Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Technical University Vienna, Faculty of Technical Chemistry, Getreidemarkt 9/163, Vienna, Austria
Michael B. Fischer
Department for Biomedical Research, Center of Experimental Medicine, Danube University Krems, Dr.-Karl-Dorrek-Straße 30, Krems an der Donau, Austria; Clinic for Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, Austria
As centre of energy production and key regulators of metabolic and cellular signaling pathways, the integrity of mitochondria is essential for mesenchymal stem cell function in tissue regeneration. Alterations in the size, shape and structural organization of mitochondria are correlated with the physiological state of the cell and its environment and could be used as diagnostic biomarkers. Therefore, high-throughput experimental and computational techniques are crucial to ensure adequate correlations between mitochondrial function and disease phenotypes.The emerge of microfluidic technologies can address the shortcomings of traditional methods to determine mitochondrial dimensions for diagnostic and therapeutic use. This review discusses optical detection methods compatible with microfluidics to measure mitochondrial dynamics and their potential for clinical stem cell research targeting mitochondrial dysfunction.
