Real-time monitoring of PARP1-dependent PARylation by ATR-FTIR spectroscopy

Annika Krüger; Alexander Bürkle; Karin Hauser; Aswin Mangerich

doi:10.1038/s41467-020-15858-w

Nature Communications (May 2020)

Real-time monitoring of PARP1-dependent PARylation by ATR-FTIR spectroscopy

Annika Krüger,
Alexander Bürkle,
Karin Hauser,
Aswin Mangerich

Affiliations

Annika Krüger: Department of Biology, University of Konstanz
Alexander Bürkle: Department of Biology, University of Konstanz
Karin Hauser: Department of Chemistry, University of Konstanz
Aswin Mangerich: Department of Biology, University of Konstanz

DOI: https://doi.org/10.1038/s41467-020-15858-w
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 15

Abstract

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The mechanism of PARP1-dependent poly-ADP-ribosylation in response to DNA damage is still under debate. Here, the authors use ATR-FTIR spectroscopy to provide time-resolved insights into the molecular details of this process under near physiological conditions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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