CXCL10 and CXCR3 in the Trigeminal Ganglion Contribute to Trigeminal Neuropathic Pain in Mice

Abstract

Yuan-Yuan Ju,1,2 Ming Jiang,2 Feifei Xu,1,3 Dongqin Wang,1,3 Bixiao Ding,1,3 Ling-Jie Ma,2 Hao Wu1 1Department of Otolaryngology, Head, and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People’s Republic of China; 2Institute of Pain Medicine, Institute of Nautical Medicine, Nantong University, Nantong, Jiangsu, People’s Republic of China; 3Medical School of Nantong University, Nantong, Jiangsu, People’s Republic of ChinaCorrespondence: Hao WuDepartment of Otolaryngology, Head, and Neck Surgery, Affiliated Hospital of Nantong University, Xisi Road 20#, Nantong, Jiangsu Province 226001, People’s Republic of ChinaEmail [email protected] MaInstitute of Pain Medicine, Institute of Nautical Medicine, Nantong University, Seyuan Road 9#, Nantong, Jiangsu Province 226019, People’s Republic of ChinaEmail [email protected]: Trigeminal neuropathic pain is very common clinically, but effective treatments are lacking. Chemokines and their receptors have been implicated in the pathogenesis of chronic pain. This study explored the role of the chemokine CXCL10 and its receptor, CXCR3, in trigeminal neuropathic pain in mice.Materials and Methods: Trigeminal neuropathic pain was established by partial infraorbital nerve ligation (pIONL) in wild-type and Cxcr3−/− mice. Facial mechanical allodynia was evaluated by behavioral testing. A lentivirus containing Cxcr3 shRNA (LV-Cxcr3 shRNA) was microinjected into the trigeminal ganglion (TG) to knock down Cxcr3 expression. Quantitative polymerase chain reaction assays and immunofluorescence staining were used to examine Cxcl10/Cxcr3 mRNA expression and protein distribution. Western blotting was performed to examine activation of extracellular signal-regulated kinase (ERK) and AKT in the TG. Intra-TG injection of an AKT inhibitor was performed to examine the role of AKT in trigeminal neuropathic pain.Results: pIONL induced persistent trigeminal neuropathic pain, which was alleviated in Cxcr3−/− mice. Intra-TG injection of LV-Cxcr3 shRNA attenuated pIONL-induced mechanical allodynia. Furthermore, pIONL increased the expression of CXCR3 and its major ligand, CXCL10, in TG neurons. Intra-TG injection of CXCL10 induced pain hypersensitivity in wild-type mice but not in Cxcr3−/− mice. CXCL10 also induced activation of ERK and AKT in the TG of wild-type mice. Finally, pIONL-induced activation of ERK and AKT was reduced in Cxcr3−/− mice. Intra-TG injection of the AKT inhibitor alleviated pIONL-induced mechanical allodynia in WT mice but not in Cxcr3−/− mice.Conclusion: CXCL10 acts on CXCR3 to induce ERK and AKT activation in TG neurons and contributes to the maintenance of trigeminal neuropathic pain.Keywords: CXCR3, CXCL10, trigeminal ganglion, neuropathic pain, mice

Keywords

• cxcr3
• cxcl10
• trigeminal ganglion
• neuropathic pain
• mice