Nature Communications (Jun 2019)

A single-nucleus RNA-sequencing pipeline to decipher the molecular anatomy and pathophysiology of human kidneys

  • Blue B. Lake,
  • Song Chen,
  • Masato Hoshi,
  • Nongluk Plongthongkum,
  • Diane Salamon,
  • Amanda Knoten,
  • Anitha Vijayan,
  • Ramakrishna Venkatesh,
  • Eric H. Kim,
  • Derek Gao,
  • Joseph Gaut,
  • Kun Zhang,
  • Sanjay Jain

DOI
https://doi.org/10.1038/s41467-019-10861-2
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 15

Abstract

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Single-cell studies in solid tissues remain challenging and have benefited from the development of single-nuclei RNA sequencing strategies. Here Lake et al. apply single-nucleus RNA sequencing to human kidney tissues to provide a comprehensive molecular and cellular atlas of the human kidney, with potential implications for the understanding of kidney physiology and disease.