Genetic Dissection of Spike Productivity Traits in the Siberian Collection of Spring Barley
Irina V. Rozanova,
Yuriy N. Grigoriev,
Vadim M. Efimov,
Alexander V. Igoshin,
Elena K. Khlestkina
Affiliations
Irina V. Rozanova
N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia
Yuriy N. Grigoriev
Siberian Research Institute of Plant Cultivation and Breeding—Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Krasnoobsk, 630501 Novosibirsk, Russia
Vadim M. Efimov
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentjeva Ave. 10, 630090 Novosibirsk, Russia
Alexander V. Igoshin
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentjeva Ave. 10, 630090 Novosibirsk, Russia
Elena K. Khlestkina
N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia
Barley (Hordeum vulgare L.) is one of the most commonly cultivated cereals worldwide. Its local varieties can represent a valuable source of unique genetic variants useful for crop improvement. The aim of this study was to reveal loci contributing to spike productivity traits in Siberian spring barley and to develop diagnostic DNA markers for marker-assisted breeding programs. For this purpose we conducted a genome-wide association study using a panel of 94 barley varieties. In total, 64 SNPs significantly associated with productivity traits were revealed. Twenty-three SNP markers were validated by genotyping in an independent sample set using competitive allele-specific PCR (KASP). Finally, fourteen markers associated with spike productivity traits on chromosomes 2H, 4H and 5H can be suggested for use in breeding programs.
