EFFECT OF LIPOPOLYSACCHARIDE OF ESCHERICHIA COLI ON PHAGOCYTE AND METABOLIC ACTIVITY OF MICE BLOOD NEUTROPHILS WITH INDUCED IMMUNE DEFICIENCY

Abstract

Aim. Experimental evaluation of effect of E. coli LPS fractions on phagocyte and metabolic activity of blood neutrophils of laboratory mice with induced immune deficiency. Materials and methods. Phagocyte activity was evaluated by phagocyte number (PN), phagocyte index (PI) and integral phagocyte index (IPI), intensity of metabolism and energetic processes of enzyme systems - by test of tetrazolium nitro blue (TNB), mean cytochemical coefficient (MCC) in spontaneous and induced tests and stimulation index (SI). Results. LPS-3 substance administration into mice with secondary immune deficiency has resulted in a significantly highest increase of PN (15.8%), IPI (17.7%), TNB-IN (10.3%), cytochemical coefficients MCC-IN (u.) and IS (u.) - 14.8 and 10.9%, respectively, compared with the parameters of immune deficient mice that had received licopid. Conclusion. Some fractions of E. coli M17 polysaccharide have immune stimulating activity.

Keywords

• e.coli
• lipopolysaccharide
• e. coli
• laboratory mice
• immune deficiency
• cyclophosphane
• phagocyte number
• phagocyte index
• tnb test
• mean cytochemical coefficient
• stimulation index