Molecules (Sep 2023)

Optical Imaging Opportunities to Inspect the Nature of Cytosolic Iron Pools

  • Robert Charles Hider,
  • Charareh Pourzand,
  • Yongmin Ma,
  • Agostino Cilibrizzi

DOI
https://doi.org/10.3390/molecules28186467
Journal volume & issue
Vol. 28, no. 18
p. 6467

Abstract

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The chemical nature of intracellular labile iron pools (LIPs) is described. By virtue of the kinetic lability of these pools, it is suggested that the isolation of such species by chromatography methods will not be possible, but rather mass spectrometric techniques should be adopted. Iron-sensitive fluorescent probes, which have been developed for the detection and quantification of LIP, are described, including those specifically designed to monitor cytosolic, mitochondrial, and lysosomal LIPs. The potential of near-infrared (NIR) probes for in vivo monitoring of LIP is discussed.

Keywords