Nature Communications (Oct 2020)
A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy
- Wenjing Yin,
- Derrick Brittain,
- Jay Borseth,
- Marie E. Scott,
- Derric Williams,
- Jedediah Perkins,
- Christopher S. Own,
- Matthew Murfitt,
- Russel M. Torres,
- Daniel Kapner,
- Gayathri Mahalingam,
- Adam Bleckert,
- Daniel Castelli,
- David Reid,
- Wei-Chung Allen Lee,
- Brett J. Graham,
- Marc Takeno,
- Daniel J. Bumbarger,
- Colin Farrell,
- R. Clay Reid,
- Nuno Macarico da Costa
Affiliations
- Wenjing Yin
- Allen Institute
- Derrick Brittain
- Allen Institute
- Jay Borseth
- Allen Institute
- Marie E. Scott
- Allen Institute
- Derric Williams
- Allen Institute
- Jedediah Perkins
- Allen Institute
- Christopher S. Own
- Voxa
- Matthew Murfitt
- Voxa
- Russel M. Torres
- Allen Institute
- Daniel Kapner
- Allen Institute
- Gayathri Mahalingam
- Allen Institute
- Adam Bleckert
- Allen Institute
- Daniel Castelli
- Allen Institute
- David Reid
- Allen Institute
- Wei-Chung Allen Lee
- Harvard Medical School
- Brett J. Graham
- Harvard Medical School
- Marc Takeno
- Allen Institute
- Daniel J. Bumbarger
- Allen Institute
- Colin Farrell
- Allen Institute
- R. Clay Reid
- Allen Institute
- Nuno Macarico da Costa
- Allen Institute
- DOI
- https://doi.org/10.1038/s41467-020-18659-3
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 12
Abstract
Electron microscopy (EM) is the gold standard for biological ultrastructure but acquisition speed is slow, making it unsuitable for large volumes. Here the authors present a parallel imaging pipeline for continuous autonomous imaging with six transmission EMs to image 1 mm3 of mouse cortex in less than 6 months.
