Easy-to-use and reliable absorbance-based MPH-GST biosensor for the detection of methyl parathion pesticide

Abstract

Due to high contamination of organophosphate (OP) insecticides in agricultural products and the environment, efficient and convenient devices for their monitoring are necessary. Here, a simple, inexpensive, efficient, and easy-to-use absorbance-based biosensor was fabricated utilizing recombinant methyl parathion hydrolase fused with glutathione-S-transferase (MPH-GST), covalently immobilized onto a chitosan film-coated polystyrene microplate, for the detection of methyl parathion (MP) as a representative of OPs. Having been connected to the transducer system designed to work through an Arduino microcontroller, the biosensor could detect MP as efficiently as the conventional methods, with the detection limit of 0.1 μM, the lowest value ever reported for this method. It was stable at 25 °C for 30 days, could function 100 rounds repetitively, and yielded high recovery with real samples. Hence, this simply designed MPH-GST biosensor could be an easy and inexpensive alternative for efficient OP screening at site to help control its contamination.

Keywords

• Absorbance-based biosensor
• Methyl parathion
• Methyl parathion hydrolase
• MPH-GST
• Organophosphate