The effect of mephedrone on human neuroblastoma and astrocytoma cells

Ibrahim M. Alanazi; Abdullah R. Alzahrani; Mohammad A. Alsaad; Abdulaziz L. Moqeem; Abdulmohsen M. Hamdi; Mohiuddin M. Taher; David G. Watson; M. Helen Grant

Saudi Pharmaceutical Journal (Apr 2024)

The effect of mephedrone on human neuroblastoma and astrocytoma cells

Ibrahim M. Alanazi,
Abdullah R. Alzahrani,
Mohammad A. Alsaad,
Abdulaziz L. Moqeem,
Abdulmohsen M. Hamdi,
Mohiuddin M. Taher,
David G. Watson,
M. Helen Grant

Affiliations

Ibrahim M. Alanazi: Department of Pharmacology and Toxicology, College of Medicine, Umm Al-Qura University, Al-Abidiyah, P.O.Box 13578, Makkah 21955, Saudi Arabia; Corresponding author.
Abdullah R. Alzahrani: Department of Pharmacology and Toxicology, College of Medicine, Umm Al-Qura University, Al-Abidiyah, P.O.Box 13578, Makkah 21955, Saudi Arabia
Mohammad A. Alsaad: Department of Parasitology, College of Medicine, Umm AL Qura University, Al-Abidiyah, P.O.Box 13578, Makkah 21955, Saudi Arabia
Abdulaziz L. Moqeem: Home Health Care,Directorate of Health Affairs Jeddah, Ministry of Health, P.O.Box11176, Ryiadh, Saudi Arabia
Abdulmohsen M. Hamdi: Home Health Care,Directorate of Health Affairs Jeddah, Ministry of Health, P.O.Box11176, Ryiadh, Saudi Arabia
Mohiuddin M. Taher: Science and Technology Unit, Deanship of Scientific Research, and Department of Medical Genetics, College of Medicine, Umm-Al-Qura University, Makkah, Saudi Arabia
David G. Watson: Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UK
M. Helen Grant: Department of Biomedical Engineering, University of Strathclyde, Glasgow G4 0NW, UK

Journal volume & issue: Vol. 32, no. 4
p. 102011

Abstract

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Mephedrone is an illegal drug that is used recreationally. Few studies have been conducted to investigate the mechanisms by which mephedrone is harming cells. In this research, we investigated the effect of mephedrone using toxicology coupled with LC-MS/MS based metabolomics in the two CNS derived cell lines. Methods of assessment such as neutral red (NR) assay, dimethylthiazolyl diphenyltetrazolium bromide (MTT), lactose dehydrogenase (LDH) measurement, and morphology were performed to identify the effect on cell viability and to identify the best concentration to be used in a metabolomics study. A concentration of 100 μM of mephedrone was used in the metabolomic experiment because at this concentration mephedrone had induced several intracellular changes. Although there no clear indicators of cellular damage caused by mephedrone. In astrocytes there was a clear indication that cell membrane function might be impaired by depletion of ether lipids.

Published in Saudi Pharmaceutical Journal

ISSN: 1319-0164 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/saudi-pharmaceutical-journal/

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