Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering

John G. Hardy; Jose Guillermo Torres-Rendon; Aldo Leal-Egaña; Andreas Walther; Helmut Schlaad; Helmut Cölfen; Thomas R. Scheibel

doi:10.3390/ma9070560

Materials (Jul 2016)

Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering

John G. Hardy,
Jose Guillermo Torres-Rendon,
Aldo Leal-Egaña,
Andreas Walther,
Helmut Schlaad,
Helmut Cölfen,
Thomas R. Scheibel

Affiliations

John G. Hardy: Lehrstuhl Biomaterialien, Universität Bayreuth, Universitätsstraße 30, Bayreuth 95447, Germany
Jose Guillermo Torres-Rendon: DWI Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, Aachen 52056, Germany
Aldo Leal-Egaña: Lehrstuhl Biomaterialien, Universität Bayreuth, Universitätsstraße 30, Bayreuth 95447, Germany
Andreas Walther: DWI Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, Aachen 52056, Germany
Helmut Schlaad: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam 14476, Germany
Helmut Cölfen: Physical Chemistry, Department of Chemistry, University of Konstanz, Universitätsstr. 10, Konstanz D-78457, Germany
Thomas R. Scheibel: Lehrstuhl Biomaterialien, Universität Bayreuth, Universitätsstraße 30, Bayreuth 95447, Germany

DOI: https://doi.org/10.3390/ma9070560
Journal volume & issue: Vol. 9, no. 7
p. 560

Abstract

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Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.

Published in Materials

ISSN: 1996-1944 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering; Technology: Engineering (General). Civil engineering (General); Science: Natural history (General): Microscopy; Science: Physics: Descriptive and experimental mechanics
Website: http://www.mdpi.com/journal/materials/

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