In this paper, the essential oil (EO) extraction from thyme by the consecutive use of ultrasound and microwave treatments is presented. The aim of this study was to apply an ultrasound pre-treatment of thyme leaves to enhance the thymol content and the extraction yield of the EO obtained by microwave-assisted hydro-distillation (MWHD). Compared with conventional hydro-distillation (CHD), the consecutive use of ultrasound pre-treatment and microwave extraction resulted in a 72% lower extraction time. When the ultrasound pre-treatment (using the ultrasonic processor with an amplitude of 70%) was applied, the EO content was 23% higher compared to the extraction without pre-treatment (2.67 ± 0.06 g EO/100 g DM for the MWHD with ultrasound pre-treatment compared with 2.18 ± 0.07 g EO/100 g DM for the MWHD without pre-treatment). The EO samples were analyzed by GC/MS. The results showed that the major component, thymol, varied from 43.54% (by CHD) to 65.94% (by the consecutive use of ultrasound and microwave treatments).
