Pseudorabies Virus Induces Viability Changes and Oxidative Stress in Swine Testis Cell-Line

Abstract

In this study, we evaluated the association between pseudorabies (PRV) virus-induced viability changes and oxidative stress in vitro cultivated swine testis (ST) cells. The kinetic of 2, 12, 24, 36 and 48 h during the cell culture with PRV by using a multiplicity of infection (MOI) of 1 TCID50 per cell were adopted. The results suggested a complex relation between cell viability and oxidative stress during PRV infection. In the early stages of PRV infection, the cell viability was higher than the control group, and the state of cellular oxidative stress remained relatively stable. After 24 h, the cell viability began to decrease, and the amount of the cellular malondialdehyde in ST cells increased significantly, and the activities of superoxide dismutase and catalase decreased significantly (P<0.05). Meanwhile, the rising concentrations of cellular hydrogen peroxide were detected prior to the changes in cell viability and oxidative stress. In conclusion, the PRV infection of ST cells leads to oxidative stress, and this stress could play a crucial role on the cell viability as the PRV infection time progresses.

Keywords

• Cell viability
• Oxidative stress
• Pseudorabies virus
• ST cell