Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Zhongying Wang; Tatyana Gurlo; Aleksey V. Matveyenko; David Elashoff; Peiyu Wang; Madeline Rosenberger; Jason A. Junge; Raymond C. Stevens; Kate L. White; Scott E. Fraser; Peter C. Butler

doi:10.1038/s42003-021-02113-1

Communications Biology (May 2021)

Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Zhongying Wang,
Tatyana Gurlo,
Aleksey V. Matveyenko,
David Elashoff,
Peiyu Wang,
Madeline Rosenberger,
Jason A. Junge,
Raymond C. Stevens,
Kate L. White,
Scott E. Fraser,
Peter C. Butler

Affiliations

Zhongying Wang: Department of Biological Sciences, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California
Tatyana Gurlo: Larry L. Hillblom Islet Research Center, David Geffen School of Medicine, University of California, Los Angeles
Aleksey V. Matveyenko: Department of Medicine, Division of Endocrinology, Metabolism, Diabetes, and Nutrition, Mayo Clinic School of Medicine
David Elashoff: Department of Biostatistics, University of California, Los Angeles David Geffen School of Medicine
Peiyu Wang: Department of Biological Sciences, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California
Madeline Rosenberger: Larry L. Hillblom Islet Research Center, David Geffen School of Medicine, University of California, Los Angeles
Jason A. Junge: Department of Biological Sciences, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California
Raymond C. Stevens: Department of Biological Sciences, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California
Kate L. White: Department of Biological Sciences, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California
Scott E. Fraser: Department of Biological Sciences, Bridge Institute, Michelson Center for Convergent Bioscience, University of Southern California
Peter C. Butler: Larry L. Hillblom Islet Research Center, David Geffen School of Medicine, University of California, Los Angeles

DOI: https://doi.org/10.1038/s42003-021-02113-1
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 11

Abstract

Read online

Using phasor-FLIM (Fluorescence Lifetime Imaging Microscopy), Wang et al. reveal differential usages of oxidative phosphorylation and glycolysis in living islet cells following glucose stimulation. This study suggests the applicability of FLIM as a drug discovery tool as it reports perturbations in glucose metabolism in living islet cells.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal