Arabian Journal of Chemistry (Jun 2021)
Novel green synthesis, chemical characterization, toxicity, colorectal carcinoma, antioxidant, anti-diabetic, and anticholinergic properties of silver nanoparticles: A chemopharmacological study
Abstract
In recent research, we compared the anti-colorectal carcinoma effects of silver nanoparticles containing AgNO3, Cannabis sativa L leaf aqueous extract and C. sativa against colorectal carcinoma in cellular models. In addition, antioxidant, anti-diabetic and anticholinergic properties of silver nanoparticles were measured. To synthesize silver nanoparticles, C. sativa leaf aqueous extract and AgNO3 solutions were combined. Chemical characterization of these nanoparticles was done with TEM, FT-IR, FE-SEM, and UV–Vis. In the FT-IR assay, more antioxidant molecules with related bonds resulted in the perfect condition for the silver reduction in silver nanoparticles. The clear peak at 425 nm wavelength in UV–Vis showed the formation of silver nanoparticles. Also, in TEM and FE-SEM images, the silver nanoparticles were spherical, with an average of 11.5 nm. MTT test was used on normal (HUVEC) and colorectal carcinoma (WiDr, SW1417 [SW-1417], DLD-1, LS123, ATRFLOX [Mutatect], and Caco-2) cell lines to determine the anti-colorectal carcinoma activities of AgNO3, C. sativa and silver nanoparticles. Silver nanoparticles had very low cell viability against colorectal carcinoma cell lines. The IC50 of C. sativa and AgNPs were 603 and 379 µg/mL against the WiDr cell line, respectively; were 500 and 285 µg/mL against the SW1417 [SW-1417] cell line, respectively; were 649 and 377 µg/mL against the DLD-1 cell line, respectively; were 634 and 350 µg/mL against the LS123 cell line, respectively; were 596 and 241 µg/mL against the ATRFLOX [Mutatect] cell line, respectively; and were 539 and 296 µg/mL against the Caco-2 cell line, respectively. The best result of the cytotoxicity characteristic of AgNPs against the above cell lines was seen in the case of ATRFLOX [Mutatect] cell line.
