Bamboo cellulose based single cell protein and nanocellulose by dilute sulfuric acid hydrolysis and fermentation

Abstract

The novel and facile multi-stage method was used for the conversion of bamboo into nanocellulose and single cell protein. Firstly, the bamboo chips were treated with sodium hydroxide solution followed by hydrogen peroxide bleaching for obtaining cellulose pulp with a brightness of 80% ISO. In the second stage, the obtained bleached cellulosic pulp was achieved with a dilute sulfuric acid and hydrogen peroxide followed by filtration for obtaining the sugar solution, which than was treated and used for Candida utilis cultivation, and nanocellulose, which than was submerged purification and refining for obtaining nanocellulose fibers. The optimal conditions of the yeast cultivation were dertermined for obtaining the single cell protein with protein content of approx. 49.5 wt.%. For isolation of nanocellulose with fiber diameter < 100 nm were proposed: concentration of hydrogen peroxide 0.25 wt.%, the concentration of sulfuric acid 0.75 wt. %, liquor to cellulose ratio 8 to 1, temperature 140oC, time 120 min. The characteristics of nanocellulose were studied by SEM, FTIR, and XRD.