Identification of non-tuberculous mycobacteria circulating in different regions of Siberia and analysis of their drug resistance

Abstract

The objective of the study: to identify the frequency of various species of non-tuberculous mycobacteria (NTM) isolated in the patients of TB services in Siberian Region during 2014-2018, to determine the correlation between rates of resistance to different antibacterial drugs.Subjects and methods. PCR hybridization on strip strips (Hain Lifescience GenoType Mycobacterium CM / AS) was used to genotype 475 NTM isolates of patients from various TB hospitals inSiberia. Drug susceptibility was tested by minimum inhibitory concentrations (MIC) on culture with serial micro-dilutions in a liquid medium using TREK Diagnostic Systems kits. 64 panels were used for slow-growing mycobacteria (SLOWMYCO), 46 - for fast-growing ones(RAPMYCO). Data processing was performed using the APL of 3.5.1 and the data analysis software of Rstudio, version 1.1.442.Results of the study1. Tomsk Region differs from the other considered regions ofSiberiadue to higher frequency of M. intracellulare among isolates identified by PCR hybridization. In other regions, M. fortuitum prevails among isolated.2. Incomparison with the reference data (CLSI M62, 2018), differences in the MIC of some NTM species isolated from patients in Siberia and theFar Eastwere observed, most likely related to the regional characteristics of these isolates.3. HTM isolates resistant to moxifloxacin are also resistant to linezolid, trimethoprim, and ciprofloxacin.4. Fast-growing clarithromycin resistant HTM isolates are predominantly sensitive to moxifloxacin, ciprofloxacin, and trimethoprim.

Keywords

• нетуберкулезные микобактерии
• изолят
• микобактериоз
• лекарственная устойчивость
• диагностика