Journal of Islamic International Medical College (Dec 2021)
Potentiation Effects of Acetylcholine at Neuromuscular Junction by Ranitidine
Abstract
Objective: To evaluate the activity of Ranitidine at neuromuscular junction with and without Pancuronium. Study Design: Experimental randomized control study. Place and Duration of Study: Department of Pharmacology, Islamic International Medical College, RIU Rawalpindi from October 2018 to September 2019. Materials and Methods: Changes in the length (contraction) of rectus abdomininis muscle of frog were recorded using students oscillograph and cumulative dose response curve with Acetylcholine was obtained (control group). The effect of Ranitidine before and after adding Pancuronium was observed using three groups. Statistical analysis of variance (ANOVA) between the groups was performed by using the student’s ‘t’ test and a P-value < 0.05 was considered statistically significant. Results: Ranitidine in a dose of 1mM concentration produced a shift of the curve to the left with mean deviation of 61.5% (SEM ± 20.5) showing an enhancement of effects of Acetylcholine. Ranitidine also produced a shift of the curve to the left in the presence of 1µg Pancuronium with the mean deviation of 104.5% (SEM ± 39.7). The shift was statistically significant (P < 0.05) showing the antagonistic effect of Ranitidine on neuromuscular junction (NMJ) blockers like Pancuronium at this concentration. Conclusion: Ranitidine in a concentration of 1mM increases the effects of Acetylcholine at neuromuscular junction (NMJ) and antagonizes the effects of NMJ blockers like Pancuronium at this concentration.
