Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain

Xueying Han; Yangkun Liu; Yan Wang; Tiejun Wang; Ning Li; Feng Hao; Lunguang Yao; Kangkang Guo

doi:10.3389/fmicb.2023.1203893

Frontiers in Microbiology (May 2023)

Isolation and characterization of porcine epidemic diarrhea virus with a novel continuous mutation in the S10 domain

Xueying Han,
Yangkun Liu,
Yan Wang,
Tiejun Wang,
Ning Li,
Feng Hao,
Lunguang Yao,
Kangkang Guo

Affiliations

Xueying Han: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China
Yangkun Liu: Henan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, China
Yan Wang: Center of Advanced Analysis & Gene Sequencing, Zhengzhou University, Zhengzhou, Henan, China
Tiejun Wang: Henan Province Engineering Technology Research Center of Animal Disease Control and Prevention, Nanyang Vocational College of Agriculture, Nanyang, Henan, China
Ning Li: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China
Feng Hao: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China
Lunguang Yao: Henan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, China
Kangkang Guo: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China

DOI: https://doi.org/10.3389/fmicb.2023.1203893
Journal volume & issue: Vol. 14

Abstract

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Porcine epidemic diarrhea virus (PEDV), which re-emerged in China in 2010, has caused severe economic losses to the global pig industry. In this study, a PEDV strain, designated PEDV WMB, was isolated from piglets with severe diarrhea on a pig farm in Henan Province of China. Whole-genome sequencing and analysis revealed that the PEDV WMB strain belongs to subtype G2c and has a unique continuous mutation in the S10 antigenic epitope of the S protein. Moreover, the virus-neutralization (VN) test indicated that polyclonal antibodies against the S10 protein of other G1 and G2 strains showed reduced VN reactivity to PEDV WMB. The pathogenicity of PEDV WMB was further investigated in 3 day-old piglets. PEDV infection-related clinical symptoms and morphological lesions were observed and confirmed by histopathological and immunohistochemical examination (IHC). These results illustrated that continuous mutation of the S10 epitope might affect the immunogenicity or pathogenicity of PEDV, providing evidence of the need to monitor the genetic diversity of the virus and develop effective measures to prevent and control PEDV.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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