A multilayered nanofibrous patch functionalized with adipose tissue extract for the treatment of bladder regeneration

Jing Wang; Chunxiang Feng; Yunpeng Zhu; Zhixian Wang; Xiang Ren; Xing Li; Yisheng Ying; Yiqun Tian; Kai Yu; Shiliang Liu; Chang Liu; Xiaoyong Zeng

Materials & Design (Aug 2022)

A multilayered nanofibrous patch functionalized with adipose tissue extract for the treatment of bladder regeneration

Jing Wang,
Chunxiang Feng,
Yunpeng Zhu,
Zhixian Wang,
Xiang Ren,
Xing Li,
Yisheng Ying,
Yiqun Tian,
Kai Yu,
Shiliang Liu,
Chang Liu,
Xiaoyong Zeng

Affiliations

Jing Wang: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Chunxiang Feng: Department of Urology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, No.106, 2nd Road of Zhongshan, Guangdong, Guangzhou 510000, China
Yunpeng Zhu: Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Zhixian Wang: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Xiang Ren: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Xing Li: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Yisheng Ying: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Yiqun Tian: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Kai Yu: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China
Shiliang Liu: Department of Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Chang Liu: Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China; Corresponding authors at: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. Jiefang Avenue, Wuhan, China (X. Zeng) Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. Jiefang Avenue, Wuhan, China (C. Liu).
Xiaoyong Zeng: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Avenue, Wuhan 430030, Hubei, China; Corresponding authors at: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. Jiefang Avenue, Wuhan, China (X. Zeng) Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. Jiefang Avenue, Wuhan, China (C. Liu).

Journal volume & issue: Vol. 220
p. 110821

Abstract

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Bladder tissue engineering is a promising approach to generating new tissues to replace damaged or diseased organs, breaking the limitations of current therapies using reconfigured autogenous bowel segments. Adipose tissue extract (ATE) is a non-cellular three-dimensional macromolecular network that provides an appropriate local microenvironment for cells and affects cellular metabolism, proliferation, differentiation, or organoid formation. This study integrated electrospun nanofibers mats and hyaluronic acid colloidal solution to constitute a multilayered nanofibrous patch, where ATE was added as a biological additive. In vitro study, we observed an increase in the pluripotent marker proteins (Oct-4, Sox-2, and Nanog) of adipose-derived stem cells (ADSCs) and bone mesenchymal stem cells (BMSCs) when cultured with ATE. The CCK-8 test, scratch assay, transwell migration assay, and tube formation assay demonstrated the positive effect of ATE on endothelial cells (ECs) proliferation, migration, and angiogenesis. The feasibility and effectiveness of the patch were investigated in a rat bladder defect model. The primary ideal results revealed the patches' great potential for tissue engineering repair and regenerative medicine.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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