Molecular detection and phylogenetic analysis of Mycoplasma bovis in cattle in Nineveh governorate, Iraq

Abstract

This study targeted to determine the prevalence of Mycoplasma bovis in cattle in Nineveh Governorate, Iraq, based on three genes, including 16S rRNA, uvrC, and gapA using polymerase chain reaction (PCR) techniques, and to investigate the phylogenetic analysis of M. bovis diagnose in the study. Various samples, including 352 blood samples, 352 nasal swabs, 40 ocular swabs, 65 synovial fluid, and 30 milk samples, were randomly obtained from 352 cattle. Based on the amplified 16S rRNA gene, the prevalence of Mycoplasma spp was 38.63% in cattle using the c-PCR technique. At the same time, there was no significant in the prevalence of M. bovis in cattle based on amplified uvrC and gapA genes, which were 30.68% and 28.69%, respectively, using the m-PCR technique. No significant difference was found between the types of samples for detecting M. bovis. The phylogenetic analysis for ten local sequences of the uvrC (5 sequence) and gapA (5 sequence) that were deposited in the NCBI GenBank under the accession numbers OR784598.1-OR784602.1 and OR792211.1-OR792215.1, with highly related 99.13-100% identity and 99.81% identity, respectively, to the other sequences that registered in the GenBank from different countries, including Canada, Egypt, Iran, Poland, and Switzerland. This study concludes that M. bovis is widespread in Nineveh Governorate, Iraq. This first study highlights the phylogenetic analysis of M. bovis in Nineveh Governorate, Iraq.

Keywords

• mycoplasma bovis
• pcr
• uvrc gene
• gapa gene
• iraq