FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver

Abstract

Summary: Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24low progenitors are less mature and have greater proliferative capacity than CD71/CD24high. We present protocols for isolation of CD71/CD24low progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24low progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids.For complete details on the use and execution of this protocol, please refer to Li et al. (2019).

Keywords

• Cell Biology
• Cell isolation
• Single Cell
• Flow Cytometry/Mass Cytometry
• Developmental biology
• Cell Differentiation