An improved method for preparation of mouse retinal cryosections

Ling Li; Yongqiong Lin; Fang Yang; Tongdan  Zou; Jialiang Yang; Houbin Zhang

doi:10.4081/ejh.2020.3154

European Journal of Histochemistry (Sep 2020)

An improved method for preparation of mouse retinal cryosections

Ling Li,
Yongqiong Lin,
Fang Yang,
Tongdan Zou,
Jialiang Yang,
Houbin Zhang

Affiliations

Ling Li: The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan
Yongqiong Lin: The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan
Fang Yang: The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan
Tongdan Zou: The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan
Jialiang Yang: The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan
Houbin Zhang: The Key Laboratory for Human Disease Gene Study of Sichuan Province and Institute of Laboratory Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan

DOI: https://doi.org/10.4081/ejh.2020.3154
Journal volume & issue: Vol. 64, no. 3

Abstract

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Immunohistochemistry using mouse retinal cryosections is a routine assay used in vision research. However, retinal tissues are fragile, and it is difficult to obtain an ideal retinal cryosection. Here, we developed a modified method for preparing retinal cryosection. Super Glue was applied on the surface of the sclera before the cornea and the lens are removed from either the unfixed or PFA-fixed mouse eyeballs. The new methods largely prevented retinal detachment in mouse retinal cryosections. Immunostaining of retinal cryosections derived from PFA-fixed mouse eyes using rod and cone markers yielded high-quality immunofluorescent images. Immunolabeling of retinal cryosections obtained from unfixed mouse eyes using a cilium-specific marker had improved orientations of photoreceptor connecting cilia. This new method substantially improves the morphology and immunostaining results of fixed and unfixed mouse eyes.

Published in European Journal of Histochemistry

ISSN: 1121-760X (Print); 2038-8306 (Online)
Publisher: PAGEPress Publications
Country of publisher: Italy
LCC subjects: Science: Biology (General)
Website: http://www.ejh.it/

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