Frontiers in Cellular and Infection Microbiology (Jul 2020)
Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria
- Peizhen Li,
- Peizhen Li,
- Peizhen Li,
- Tingyuan Zhu,
- Tingyuan Zhu,
- Tingyuan Zhu,
- Danying Zhou,
- Danying Zhou,
- Wei Lu,
- Wei Lu,
- Hongmao Liu,
- Hongmao Liu,
- Zhewei Sun,
- Zhewei Sun,
- Jun Ying,
- Jun Ying,
- Junwan Lu,
- Junwan Lu,
- Xi Lin,
- Xi Lin,
- Xi Lin,
- Kewei Li,
- Kewei Li,
- Kewei Li,
- Jianchao Ying,
- Jianchao Ying,
- Jianchao Ying,
- Qiyu Bao,
- Qiyu Bao,
- Qiyu Bao,
- Teng Xu,
- Teng Xu
Affiliations
- Peizhen Li
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Peizhen Li
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Peizhen Li
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Tingyuan Zhu
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Tingyuan Zhu
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Tingyuan Zhu
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Danying Zhou
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Danying Zhou
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Wei Lu
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Wei Lu
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Hongmao Liu
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Hongmao Liu
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Zhewei Sun
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Zhewei Sun
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Jun Ying
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Jun Ying
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Junwan Lu
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Junwan Lu
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Xi Lin
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Xi Lin
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Xi Lin
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Kewei Li
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Kewei Li
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Kewei Li
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Jianchao Ying
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Jianchao Ying
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Jianchao Ying
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Qiyu Bao
- Key Laboratory of Medical Genetics of Zhejiang Province, Key Laboratory of Laboratory Medicine, Ministry of Education, Wenzhou Medical University, Wenzhou, China
- Qiyu Bao
- School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
- Qiyu Bao
- Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, China
- Teng Xu
- Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
- Teng Xu
- Institute of Translational Medicine, Baotou Central Hospital, Baotou, China
- DOI
- https://doi.org/10.3389/fcimb.2020.00369
- Journal volume & issue
-
Vol. 10
Abstract
Bacterial resistance to antibiotics has become an important concern for public health. This study was aimed to investigate the characteristics and the distribution of the florfenicol-related resistance genes in bacteria isolated from four farms. A total of 106 florfenicol-resistant Gram-negative bacilli were examined for florfenicol-related resistance genes, and the positive isolates were further characterized. The antimicrobial sensitivity results showed that most of them (100, 94.33%) belonged to multidrug resistance Enterobacteriaceae. About 91.51% of the strains carried floR gene, while 4.72% carried cfr gene. According to the pulsed-field gel electrophoresis results, 34 Escherichia coli were subdivided into 22 profiles, the genetic similarity coefficient of which ranged from 80.3 to 98.0%. The multilocus sequence typing (MLST) results revealed 17 sequence types (STs), with ST10 being the most prevalent. The genome sequencing result showed that the Proteus vulgaris G32 genome consists of a 4.06-Mb chromosome, a 177,911-bp plasmid (pG32-177), and a 51,686-bp plasmid (pG32-51). A floR located in a drug-resistant region on the chromosome of P. vulgaris G32 was with IS91 family transposase, and the other floR gene on the plasmid pG32-177 was with an ISCR2 insertion sequence. The cfr gene was located on the pG32-51 flanked by IS26 element and TnpA26. This study suggested that the mobile genetic elements played an important role in the replication of resistance genes and the horizontal resistance gene transfer.
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