ADAPTABILITY AND PHYSIO-CHEMICAL CHARACTERISATION OF CELL CULTURE ADAPTED VACCINE STRAIN OF DUCK PLAGUE VIRUS

Abstract

Vaccine strain of DPV produced typical lesions on chicken embryo and characteristic CPE in CEF cell culture like rounding and clumping of cells, cytoplasmic vacuolations, bridge formation, syncytium formation and intranuclear inclusion bodies. When the virus passaged in CEF the time required for appearance of CPE reduced as the number of passages progressed. Similarly titre of virus also increased as the passages increased which indicate adaptation of the virus to the CEF cell culture. The 5''' and 10'h passage viruses, completely inactivated at 56°C for 30 min. and lost its infectivity at the pH 3 and 11 but was stable at pH 7.2, indicated CEF passages of virus did not influence the physico-chemical property of virus