Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)

Abstract

Summary: Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens.For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).

Keywords

• Health Sciences
• Clinical Protocol
• Immunology
• Metabolomics
• Mass Spectrometry