A novel composite membrane pectin from Cyclea Barbata Miers blend with chitosan for accelerated wound healing

Abstract

In this work, pectin derived from Cyclea Barbata Miers (CBM) was successfully blended with chitosan as a novel membrane and the physico-chemical analysis was investigated. Based on SEM analysis revealed that the chitosan/pectin blended membrane showed a rough surface texture compared to pristine chitosan with smooth and uniform membran. In addition, fourier-transform infrared spectroscopy (FTIR) analysis indicates that chitosan and pectin were successfully crosslinked, meanwhile x-ray diffraction (XRD) analysis found that pectin extracted from CBM exhibited crystalline structure. Thermal analysis based on thermogravimetric analysis (TGA) suggesting that chitosan/pectin blend membrane exhibited significant improvement in the thermal stability with residual mass of 54% at 360 °C. Furthermore, swelling degree, water vapor transmission rate (WVTR) and porosity of the chitosan/pectin blend membrane were 266%, 9.68 g/m2h, and 64%, respectively. Most importantly, in-vivo results indicated that the prepared membrane of chitosan/pectin immobilized with Musa Paradisiaca Linn (MPL) group had better wound closure with better wound reduction percentage than the gauze-treated wound (the control group). This finding revealed that the chitosan/pectin blend membrane could paves the way for future biomedical wound dressing application.

Keywords

• Chitosan
• Pectin
• Cyclea Barbata Miers
• Membrane
• Wound healing