Content Determination and Chemical Clustering Analysis of Tanshinone and Salvianolic Acid in <i>Salvia</i> spp.
Feiyan Wang,
Yufeng Bao,
Furui Yang,
Lu Yuan,
Xinchun Han,
Yanbo Huang,
Yukun Wei,
Lei Zhang,
Zongqi Yang,
Dongfeng Yang
Affiliations
Feiyan Wang
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Yufeng Bao
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Furui Yang
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Lu Yuan
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Xinchun Han
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Yanbo Huang
Eastern China Conservation Centre for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai 200120, China
Yukun Wei
Shanghai Botanical Garden/Shanghai Engineering Research Centre of Sustainable Plant Innovation, Shanghai 201600, China
Lei Zhang
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Zongqi Yang
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Dongfeng Yang
Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
Salvia miltiorrhiza is one of the famous traditional Chinese medicines for treating cardiovascular and cerebrovascular diseases. Tanshinone and phenolic acids are the main active compounds of Salvia miltiorrhiza, whereas the distribution patterns of the two kinds of components are still unclear among Salvia spp. In this work, high-performance liquid chromatography was applied to analyze the distribution patterns of major components in the roots and leaves of 58 Salvia spp. The results showed that the distribution patterns of tanshinone and phenolic acids in Salvia spp. varied significantly. Phenolic acid components such as rosmarinus acid, caffeic acid, and danshensu are widely distributed in the roots and leaves, and phenolic acids in the leaves of Salvia spp. are generally higher than that in roots. Tanshinones are mainly detected in the roots of Salvia przewalskii, Salvia trijuga, Salvia castanea, and Salvia yunnanensis. The content of major components of the different species varied significantly. The content of phenolic acids in most Salvia spp. generally followed the pattern of salvianolic acid B > rosmarinic acid > danshensu > caffeic acid both in the roots and leaves. Tanshinone IIA and cryptotanshinone were the main lipophilic components of Salvia spp. distributed in southwest China. A correlation between the distribution pattern of tanshinone and the genetic relationship of species was indicated in the work. This research systematically reveals the distribution patterns of tanshinone and phenolic acids in Salvia spp., providing a theoretical basis for the development and utilization of medicinal resources of Salvia.
