A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

Michael W. Martynowycz; Anna Shiriaeva; Max T. B. Clabbers; William J. Nicolas; Sara J. Weaver; Johan Hattne; Tamir Gonen

doi:10.1038/s41467-023-36733-4

Nature Communications (Feb 2023)

A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

Michael W. Martynowycz,
Anna Shiriaeva,
Max T. B. Clabbers,
William J. Nicolas,
Sara J. Weaver,
Johan Hattne,
Tamir Gonen

Affiliations

Michael W. Martynowycz: Howard Hughes Medical Institute, University of California
Anna Shiriaeva: Howard Hughes Medical Institute, University of California
Max T. B. Clabbers: Howard Hughes Medical Institute, University of California
William J. Nicolas: Howard Hughes Medical Institute, University of California
Sara J. Weaver: Howard Hughes Medical Institute, University of California
Johan Hattne: Howard Hughes Medical Institute, University of California
Tamir Gonen: Howard Hughes Medical Institute, University of California

DOI: https://doi.org/10.1038/s41467-023-36733-4
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 15

Abstract

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Here, authors demonstrate a method for milling vitrified biological material. Using correlative fluorescence and electron microscopy images, MicroED data is collected for the adenosine receptor.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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