Effects of Dietary <i>Lentinus edodes</i> Fermentation Supplementation on Digestive Enzyme Activity, Antioxidant Capacity and Morphology of the Liver and Intestine in Largemouth Bass (<i>Micropterus salmoides</i>) Fed High Plant Protein Diets
Jiaming Xu,
Zhoulin Yu,
Guangye Liu,
Sijie Li,
Guoyong Zhou,
Hanhua Wang,
Yewei Dong,
Cuihong You,
Weidong Bai,
Meng Zhou,
Yanhua Huang,
Xiaohong Tan
Affiliations
Jiaming Xu
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Zhoulin Yu
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Guangye Liu
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Sijie Li
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Guoyong Zhou
YueHao Biotechnology (Guangzhou) Co., Ltd., Guangzhou 510663, China
Hanhua Wang
YueHao Biotechnology (Guangzhou) Co., Ltd., Guangzhou 510663, China
Yewei Dong
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Cuihong You
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Weidong Bai
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou 510225, China
Meng Zhou
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Yanhua Huang
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Xiaohong Tan
Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
This study evaluated the effect of Lentinus edodes fermentation (LEF) on digestive enzyme activity, antioxidant capacity and morphology of the liver and intestine in largemouth bass (Micropterus salmoides) fed high plant protein diets (HPPD). LEF was supplemented in HPPD with 0 g kg−1 (LEF0), 10 g kg−1 (LEF1), 20 g kg−1 (LEF2), 30 g kg−1 (LEF3), 40 g kg−1 (LEF4), 50 g kg−1 (LEF5), respectively, and then the six diets were fed to largemouth bass with a body weight of 28.8 ± 0.05 g for eight weeks. Juvenile fish were randomized into 6 groups and each group had 4 replicates with 40 fish. Dietary LEF supplementation alleviated the liver inflammatory reaction of largemouth bass caused by HPPD and improved liver morphology. Goblet cells multiplied and the gut muscle layer thickened after LEF supplementation. The LEF significantly increased amylase activity in the liver and intestine of largemouth bass in individual experimental groups. The LEF could increase the activity of catalase in the liver and intestine of largemouth bass (p p < 0.05). Dietary LEF supplementation had no significant effect on the intestinal flora of largemouth bass. These findings imply that LEF supplementation can reduce liver inflammation, enhance intestinal tissue morphology, and eventually benefit largemouth bass health.
