Knockdown of FOXRED2 restrains proliferation, invasion and migration of human melanoma cells
Mujin Li,
Si Qin,
Zhijun Xu,
Yingjun Feng,
Sihui Li,
Xianwen Li,
Zhenyu Lu,
Shuting Huang,
Siman Shi,
Yixue Duan,
Ju Wen
Affiliations
Mujin Li
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China; Shenshan Medical Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Shanwei, 516621, China
Si Qin
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Zhijun Xu
Shenshan Medical Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Shanwei, 516621, China
Yingjun Feng
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Sihui Li
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Xianwen Li
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Zhenyu Lu
Department of Dermatology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510799, China
Shuting Huang
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Siman Shi
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Yixue Duan
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China
Ju Wen
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China; Corresponding author. Guangdong Second Provincial General Hospital, No. 466 Xingang Middle Road, Haizhu District, Guangzhou, 510317, China.
Objectives: This experiment investigated the role of the FAD-dependent oxidoreductase domain-containing 2 (FOXRED2) in the development of cutaneous malignant melanoma. Methods: We explored the expression and prognostic effects of FOXRED2 in cutaneous malignant melanoma by performing bioinformatics analyses and immunohistochemical staining experiments and verified the biological influence of FOXRED2 on human melanoma cells using in vitro experiments. Results: FOXRED2 expression was significantly higher in cutaneous malignant melanoma compared to normal skin and nevus tissues and closely associated with prognosis. The expression levels of FOXRED2 mRNA and protein were significantly upregulated in human melanoma cell lines, and knocking down FOXRED2 expression inhibits proliferation, invasion, and migration, promotes apoptosis, and alters tumor cell biology in A2058 and A375 cells. Conclusion: FOXRED2 may play a crucial role in the development and progression of cutaneous malignant melanoma.
