ST Genesia reference values of 117 healthy donors measured with STG‐BleedScreen, STG‐DrugScreen and STG‐ThromboScreen reagents

Abstract

Abstract Introduction The ST Genesia is a benchtop, fully automated thrombin generation (TG) device. It is completely standardized and ensures a uniform heat distribution throughout the measurement. We aimed to determine reference values and to compare TG in men and women with and without the use of oral contraceptives (OCs). Materials and Methods Plasma from 117 healthy donors was measured on the ST Genesia with the available reagent kits: STG‐BleedScreen, STG‐DrugScreen, and STG‐ThromboScreen. All kits include at least two quality controls and a reference plasma to normalize data. STG‐ThromboScreen has a second trigger containing thrombomodulin (TM) to include the effect on the protein C pathway. Means were compared with one‐way analysis of variance and reference ranges were established with 2.5th to 97.5th percentiles on absolute TG parameters. Results Mean age of the donors was 35 years (SD ± 12); 49.6% were men, 37.6% women without OCs, and 12.8% women with OCs. Men and women without OCs had, respectively, a mean peak height of 167 nM and 164 nM with STG‐BleedScreen, 335 nM and 351 nM with STG‐DrugScreen, and 192 nM and 198 nM with STG‐ThromboScreen. Women taking OCs had a mean peak height of 263 nM, 473 nM, and 312nM, respectively (P < .05 compared to men/women without OCs). TM decreased endogenous thrombin potential by 54% in men, 47% in women without OCs, and only 25% in women with OCs (P < .05 compared to men/women without OCs). Conclusions TG in men and women without OCs was similar; however, women taking OCs had significantly higher TG values, and the effect of TM was also less pronounced in these women.

Keywords

• oral contraceptives
• protein C
• reference values
• thrombin
• thrombomodulin