Nature Communications (Oct 2022)
De novo analysis of bulk RNA-seq data at spatially resolved single-cell resolution
- Jie Liao,
- Jingyang Qian,
- Yin Fang,
- Zhuo Chen,
- Xiang Zhuang,
- Ningyu Zhang,
- Xin Shao,
- Yining Hu,
- Penghui Yang,
- Junyun Cheng,
- Yang Hu,
- Lingqi Yu,
- Haihong Yang,
- Jinlu Zhang,
- Xiaoyan Lu,
- Li Shao,
- Dan Wu,
- Yue Gao,
- Huajun Chen,
- Xiaohui Fan
Affiliations
- Jie Liao
- College of Pharmaceutical Sciences, Zhejiang University
- Jingyang Qian
- College of Pharmaceutical Sciences, Zhejiang University
- Yin Fang
- College of Computer Science and Technology, Zhejiang University
- Zhuo Chen
- College of Computer Science and Technology, Zhejiang University
- Xiang Zhuang
- College of Computer Science and Technology, Zhejiang University
- Ningyu Zhang
- College of Computer Science and Technology, Zhejiang University
- Xin Shao
- College of Pharmaceutical Sciences, Zhejiang University
- Yining Hu
- College of Pharmaceutical Sciences, Zhejiang University
- Penghui Yang
- College of Pharmaceutical Sciences, Zhejiang University
- Junyun Cheng
- College of Pharmaceutical Sciences, Zhejiang University
- Yang Hu
- College of Pharmaceutical Sciences, Zhejiang University
- Lingqi Yu
- College of Pharmaceutical Sciences, Zhejiang University
- Haihong Yang
- College of Computer Science and Technology, Zhejiang University
- Jinlu Zhang
- College of Pharmaceutical Sciences, Zhejiang University
- Xiaoyan Lu
- College of Pharmaceutical Sciences, Zhejiang University
- Li Shao
- Institute of Translational Medicine, The Affiliated Hospital of Hangzhou Normal University, Hangzhou Normal University
- Dan Wu
- College of Biomedical Engineering and Instrument Science, Zhejiang University
- Yue Gao
- Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine
- Huajun Chen
- College of Computer Science and Technology, Zhejiang University
- Xiaohui Fan
- College of Pharmaceutical Sciences, Zhejiang University
- DOI
- https://doi.org/10.1038/s41467-022-34271-z
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 19
Abstract
Current methods to reanalyze bulk RNA-seq at spatially resolved single-cell resolution have limitations. Here, the authors develop Bulk2Space, a spatial deconvolution algorithm using single-cell and spatial transcriptomics as references, providing new insights into spatial heterogeneity within bulk tissue.
