Use of X-ray microprobe to diagnose bone tissue demineralization after caffeine administration Use of X-ray microprobe to diagnose bone tissue demineralization after caffeine administration

Abstract

Caffeine is a methylxanthine which permeates the placenta. In studies on animals, it has been<br />shown to produce teratogenic and embryotoxic effects in large doses. The objective of this study was to<br />assess the influence of caffeine on the development of bone tissue, with particular reference to elemental<br />bone composition using an X-ray microprobe. The research was conducted on rats. The fertilized females<br />were randomly divided into an experimental and a control group. The experimental group was<br />given caffeine orally in 30 mg/day doses from the 8th to the 21st day of pregnancy, while the control group<br />was given water. The fetuses were used to assess the growth and mineralization of the skeleton. On the<br />basis of double dyeing, a qualitative analysis of the bone morphology and mineralization was conducted.<br />For calcium and potassium analysis, an X-ray microprobe was used. In 67 fetuses from the experimental<br />group, changes in skeleton staining with the alcian-alizarin method were noticed. The frequency of the<br />development of variants in the experimental group was statistically higher. In the experimental group,<br />a significant decrease in the calcium level, as well as an increase in the potassium level, was observed.<br />The X-ray microprobe’s undoubted advantage is that is offers a quick qualitative and quantitative analysis<br />of the elemental composition of the examined samples. Employing this new technique may furnish us<br />with new capabilities when investigating the essence of the pathology process.Caffeine is a methylxanthine which permeates the placenta. In studies on animals, it has been<br />shown to produce teratogenic and embryotoxic effects in large doses. The objective of this study was to<br />assess the influence of caffeine on the development of bone tissue, with particular reference to elemental<br />bone composition using an X-ray microprobe. The research was conducted on rats. The fertilized females<br />were randomly divided into an experimental and a control group. The experimental group was<br />given caffeine orally in 30 mg/day doses from the 8th to the 21st day of pregnancy, while the control group<br />was given water. The fetuses were used to assess the growth and mineralization of the skeleton. On the<br />basis of double dyeing, a qualitative analysis of the bone morphology and mineralization was conducted.<br />For calcium and potassium analysis, an X-ray microprobe was used. In 67 fetuses from the experimental<br />group, changes in skeleton staining with the alcian-alizarin method were noticed. The frequency of the<br />development of variants in the experimental group was statistically higher. In the experimental group,<br />a significant decrease in the calcium level, as well as an increase in the potassium level, was observed.<br />The X-ray microprobe’s undoubted advantage is that is offers a quick qualitative and quantitative analysis<br />of the elemental composition of the examined samples. Employing this new technique may furnish us<br />with new capabilities when investigating the essence of the pathology process.