Combined enzymatic degradation of dextran and starch towards enhancement of the raw cane sugar juice filtration efficiency

Abstract

By assessing the polarization, colour, dextran, starch, phenols, proteins, melassigenic cations (Na+, K+) and Ca2+, reducing sugars and pH, presented study evaluates the main factors influencing non-sugars content and juice filterability in 17 raw cane sugar samples of different geographical origin. α-amylase and dextranase were applied for combined enzymatic degradation of starch and dextran as main undesirable compounds in raw cane sugar juices (15 °Brix and pH 5.5) at four different concentrations (10:12.5, 20:25, 30:37.5 and 40:50 ppm) and 55 °C during 30 min. Decrease of starch and dextran concentrations in juices remarkably increased filterability in range of 20-75% for different raw cane sugar juices. Combined enzymatic effect of α-amylase and dextranase mixture in concentration 30:37.5 ppm showed best result for improving filterability of raw cane sugar juices through reduction in starch and dextran content as main non-sugar impurities. Selecting the raw cane sugar with appropriate quality parameters facilitates the refining process. Simultaneous addition of α-amylase and dextranase mixture (30:37.5 ppm/juice) can improve filterability of low polarization raw cane sugar juice up to 41% and contribute to the refining process enhancement and thus obtention of high-quality refined cane sugar.

Keywords

• raw sugars
• filterability
• enzymatic treatment
• dextranase
• α-amylase