On-site applicable diagnostic fluorescent probe for fire blight bacteria
Yuna Jung,
Ji Hye Jin,
Minji Jung,
In Sun Hwang,
Nguyen Trung Vu,
Yoon Kyung Park,
Na Hee Kim,
Ki-Ho Park,
Chang-Sik Oh,
Dokyoung Kim
Affiliations
Yuna Jung
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
Ji Hye Jin
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
Minji Jung
Medical Science Research Institute, Kyung Hee University, Seoul 02447, Republic of Korea
In Sun Hwang
Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea
Nguyen Trung Vu
Graduate School of Green-Bio Science, Kyung Hee University, Yongin 17104, Republic of Korea
Yoon Kyung Park
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
Na Hee Kim
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
Ki-Ho Park
Division of Infectious Diseases, Department of Internal Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
Chang-Sik Oh
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea
Dokyoung Kim
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul 02447, Republic of Korea; Center for Converging Humanities, Kyung Hee University, Seoul 02447, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea; UC San Diego Materials Research Science and Engineering Center, 9500 Gilman Drive, La Jolla, CA 92093, USA; Corresponding author
Summary: Fire blight is a representative plant infection that contaminates edible plants and causes socio-economic problems in agricultural and livestock industries globally. It is caused by the pathogen Erwinia amylovora (E. amylovora) creates lethal plant necrosis and spreads rapidly across plant organs. We newly disclose the fluorogenic probe B-1 for real-time on-site detection of fire blight bacteria for the first time. B-1 exhibited no emission signals but manifested bright emission properties in the presence of fire blight bacteria. Based on these features, fluorescence imaging of the fire blight bacteria and its real-time detection from the infected host plant tissues were conducted. The detection limit against E. amylovora was 102 CFU/mL, which had excellent sensitivity. The fluorogenic probe-based on-site diagnostic technology was supplemented by introducing a new portable UV device. This work holds enormous potential to be a new advanced tool for detecting fire blight in agricultural and livestock industries.
