The Development of a High-Throughput Homonuclear Decoupling HSQC NMR Platform for the Determination of 10 Sex Hormones in Animal-Source Food and Medicines
Bing Wang,
Qing-Zhi Liu,
Jing-Ya Yang,
Yu-Jie Du,
Nai-Shuo Liu,
Wei-Liang Cui,
Man Yuan,
Yong Zhang,
Jing-Qi Wang,
Dong-Liang Wang,
Shu-Qi Wang
Affiliations
Bing Wang
Shandong Institute for Food and Drug Control, Jinan 250101, China
Qing-Zhi Liu
Pharmaceutical College, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Jing-Ya Yang
The First Clinical School, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Yu-Jie Du
Pharmaceutical College, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Nai-Shuo Liu
School of Pharmaceutical Science, Shandong University, Jinan 250012, China
Wei-Liang Cui
Shandong Institute for Food and Drug Control, Jinan 250101, China
Man Yuan
Hebei Edible Bird’s Nest Fresh Stew Technology Innovation Center, Langfang 065700, China
Yong Zhang
Hebei Edible Bird’s Nest Fresh Stew Technology Innovation Center, Langfang 065700, China
Jing-Qi Wang
Hebei Edible Bird’s Nest Fresh Stew Technology Innovation Center, Langfang 065700, China
Dong-Liang Wang
Hebei Edible Bird’s Nest Fresh Stew Technology Innovation Center, Langfang 065700, China
Shu-Qi Wang
School of Pharmaceutical Science, Shandong University, Jinan 250012, China
Owing to their endocrine disruption effect, the hormone levels in animal-source food and medicines need to be efficiently and accurately quantified by a reliable analytical method. In the current study, by using a homonuclear decoupling and heteronuclear single quantum correlation (HSQC) experiment, coupled with non-uniform sampling (NUS) that was used to shorten the experimental time, we developed a method to quantify 10 hormone residues in animal-source products. This method was validated following the guidelines of USP–NF 2022. The application of the homonuclear decoupling (HD) technique to conventional HSQC yielded 2D spectra that exhibited excellent signal separation and specificity. For all the tested hormones, good linearity with correlation coefficients of more than 0.99 was observed in the linear range of 0.2–6 mg/0.6 mL. Satisfactory precision and recoveries of spiked animal samples were also obtained. Finally, the method was applied in residue determination of 10 hormones in real animal-source samples at the ug/g level.
