Food and Agricultural Immunology (Jan 2018)

Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

  • Jingjing Li,
  • Aimin Zhi,
  • Guochao Jia,
  • Hui Nie,
  • Lianzhong Ai

DOI
https://doi.org/10.1080/09540105.2017.1364706
Journal volume & issue
Vol. 29, no. 1
pp. 147 – 158

Abstract

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In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.

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