Effects of starvation and subsequent re-feeding on intestinal microbiota, and metabolic responses in Nile tilapia, Oreochromis niloticus

Michael Essien Sakyi; Jia Cai; Jufen Tang; Emmanuel Delwin Abarike; Liqun Xia; Pengfei Li; Felix Kofi Agbeko Kuebutornye; Zihong Zou; Zhenyu Liang; Jichang Jian

Aquaculture Reports (Jul 2020)

Effects of starvation and subsequent re-feeding on intestinal microbiota, and metabolic responses in Nile tilapia, Oreochromis niloticus

Michael Essien Sakyi,
Jia Cai,
Jufen Tang,
Emmanuel Delwin Abarike,
Liqun Xia,
Pengfei Li,
Felix Kofi Agbeko Kuebutornye,
Zihong Zou,
Zhenyu Liang,
Jichang Jian

Affiliations

Michael Essien Sakyi: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China
Jia Cai: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China; Guangxi Key Lab for Marine Natural Products and Combinational Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Centre, Guangxi Academy of Sciences, Nanning, 530007, PR China; Shenzhen Research Institute of Guangdong Ocean University, PR China; Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science Technology, Qingdao, PR China; Corresponding authors at: Fisheries College, Guangdong Ocean University, No. 1 of Haida Road, Mazhang District, Zhanjiang, 524088, Guangdong Province, PR China.
Jufen Tang: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China; Corresponding authors at: Fisheries College, Guangdong Ocean University, No. 1 of Haida Road, Mazhang District, Zhanjiang, 524088, Guangdong Province, PR China.
Emmanuel Delwin Abarike: Department of Fisheries and Aquatic Resources Management, University for Development Studies, Tamale, Ghana
Liqun Xia: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China; Shenzhen Research Institute of Guangdong Ocean University, PR China
Pengfei Li: Guangxi Key Lab for Marine Natural Products and Combinational Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Centre, Guangxi Academy of Sciences, Nanning, 530007, PR China
Felix Kofi Agbeko Kuebutornye: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China
Zihong Zou: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China
Zhenyu Liang: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China
Jichang Jian: College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Zhanjiang, 524088, PR China; Key Laboratory of Control for Diseases of Aquatic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, PR China; Guangxi Key Lab for Marine Natural Products and Combinational Biosynthesis Chemistry, Guangxi Beibu Gulf Marine Research Centre, Guangxi Academy of Sciences, Nanning, 530007, PR China; Shenzhen Research Institute of Guangdong Ocean University, PR China; Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science Technology, Qingdao, PR China

Journal volume & issue: Vol. 17
p. 100370

Abstract

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The effects of starvation and re-feeding on Nile tilapia (Oreochromis niloticus) remain incompletely understood so far. In this study, the changes of intestinal microbiota, the metabolic and immune (antioxidant) responses of Nile tilapia (Oreochromis niloticus) during starvation and re-feeding stages were investigated. The metabolic responses (cortisol, ALT, AST, and leptin) and immune responses (hsp70, SOD, and CAT) were measured. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the starvation group significantly increased during starvation and decrease after re-feeding. Cortisol levels increase during starvation and decreased after re-feeding. During starvation, the mRNA expression of superoxide dismutase (SOD), catalase (CAT), and heat shock protein (hsp70) were up-regulated while leptin was down-regulated. The microbial taxonomic composition of the starvation group slightly increased from the feeding and re-feeding groups. The relative abundance of the top five bacterial phyla in the intestinal of Nile tilapia (feeding, starvation and re-feeding groups) were Fusobacteria, Proteobacteria, Firmicutes, Bacteriodetes, and Actinobacteria. The relative abundance of the top three intestinal bacterial genera of Nile tilapia (feeding, starvation and re-feeding groups) were Cetobacterium, Romboutsia, Plesiomonas, Bacteroides, and Clostridium senso stricto. Taken together, starvation and re-feeding can modulate the diversity of microbial communities, the metabolic and immune responses of Nile tilapia.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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