Improvement of determination of sterols in vegetable oils by gas chromatography

Abstract

On the basis of national standard method GB/T25223—2010, the method of determining sterols in animal and vegetable oils and fats by gas chromatography was improved and the preparation process of neutral aluminum oxide column was modified. The solvent used for washing sterol in silica gel was replaced by acetone, the derivatization reagent was replaced by 99% BSTFA+ 1% TMCS. The results showed that: The improved method was easier to operate than the national standard method, with high response value and low cost, but longer time.

Keywords

• sterol
• gas chromatography
• detect
• improvement