Wound healing potential of green synthesized silver nanoparticles of Glycyrrhiza glabra linn root extract: A preclinical study

Abstract

Background: The research emphasizes the eco-friendly and sustainable approach of nanoparticle synthesis, leveraging the medicinal properties inherent in Glycyrrhiza glabra extract, which is known for its antimicrobial, anti-inflammatory, and antioxidant properties. By combining the therapeutic potential of the plant extract with the wound-healing capabilities of silver nanoparticles, the study seeks to develop an innovative, biocompatible, and effective treatment for enhanced wound healing process in excision and incision rat model. Methods: Glycyrrhiza glabra Linn root was extracted with alcohol and utilized in the green synthesis of silver nanoparticles. The green synthesized nanoparticles were characterized, formulated into gel, and evaluated for their potential to heal wound in rat wound model. Results: The results of the study showed that the detection of ultra-violet visible spectroscopy peaks at 412 nm and 403 nm. The presence of tannins, saponins, flavonoids, and phenols in plant extract caused the capping and bio-reduction of silver, as demonstrated by FTIR. Silver nanoparticles that were biosynthesized were in between 69.7 and 419.1 nm in size. The study's formulation of synthesized silver nanoparticles demonstrated significant wound healing potential by promoting wound contraction and accelerating healing after 21 days of study, which was demonstrated by histopathological evaluation and complete epithelialization with regenerated hair follicles and higher collagen tissue. Conclusion: The current study found that the biomolecules of Glycyrrhiza glabra Linn root alcoholic extract, which are responsible for augmenting the plant extract's capacity to heal wounds, were capped on silver nanoparticles.

Keywords

• Glycyrrhiza glabra
• Silver nanoparticles
• Wound