The Curcumin Supplementation with Piperine Can Influence the Acute Elevation of Exercise-Induced Cytokines: Double-Blind Crossover Study
Stéfani Miranda-Castro,
Felipe J. Aidar,
Samara Silva de Moura,
Lucas Marcucci-Barbosa,
Lázaro Fernandes Lobo,
Francisco de Assis Dias Martins-Júnior,
Roberta da Silva Filha,
Pedro Alves Soares Vaz de Castro,
Ana Cristina Simões e Silva,
Danielle da Glória de Souza,
Siomara Aparecida da Silva,
Kelerson Mauro de Castro Pinto,
Guilherme de Paula Costa,
Ana Filipa Silva,
Filipe Manuel Clemente,
William Valadares Campos Pereira,
Albená Nunes-Silva
Affiliations
Stéfani Miranda-Castro
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Felipe J. Aidar
Department of Physical Education, Federal University of Sergipe (UFS), São Cristóvão 49100-000, Brazil
Samara Silva de Moura
Graduate Health and Nutrition Program, Federal University of Ouro Preto (PPGSN/UFOP), Ouro Preto 35400-000, Brazil
Lucas Marcucci-Barbosa
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Lázaro Fernandes Lobo
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Francisco de Assis Dias Martins-Júnior
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Roberta da Silva Filha
Interdisciplinary Laboratory of Medical Investigation, Department of Pediatrics, Faculty of Medicine UFMG (LIIM/UFMG), Belo Horizonte 30130-100, Brazil
Pedro Alves Soares Vaz de Castro
Interdisciplinary Laboratory of Medical Investigation, Department of Pediatrics, Faculty of Medicine UFMG (LIIM/UFMG), Belo Horizonte 30130-100, Brazil
Ana Cristina Simões e Silva
Interdisciplinary Laboratory of Medical Investigation, Department of Pediatrics, Faculty of Medicine UFMG (LIIM/UFMG), Belo Horizonte 30130-100, Brazil
Danielle da Glória de Souza
Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil
Siomara Aparecida da Silva
Sports Teaching Methodology Laboratory, School of Physical Education, Federal University of Ouro Preto (LAMEES/EEF-UFOP), Ouro Preto 35400-000, Brazil
Kelerson Mauro de Castro Pinto
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Guilherme de Paula Costa
Graduate Program in Biological Sciences, Research Center in Biological Sciences, Federal University of Ouro Preto (CEBIOL), Ouro Preto 35400-000, Brazil
Ana Filipa Silva
Sports and Leisure School, Polytechnic Institute of Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347 Viana do Castelo, Portugal
Filipe Manuel Clemente
Sports and Leisure School, Polytechnic Institute of Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347 Viana do Castelo, Portugal
William Valadares Campos Pereira
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Albená Nunes-Silva
Laboratory of Exercise Inflammation and Immunology, School of Physical Education, Federal University of Ouro Preto (LABIIEX/EEF-UFOP), Ouro Preto 35400-000, Brazil
Background: to evaluate the effects of one week of supplementation with curcumin combined with piperine on physical performance, immune system cell counts, muscle damage, and plasma levels of inflammatory markers after a treadmill running training session. Methods: This study is a double-blind, crossover-balanced clinical trial with a three-week intervention. Sixteen male runners with a mean age of 36 ± 9 years and VO2 max of 60.6 ± 9.03 mL.kg −1 min −1 were recruited and randomly divided into 2 groups: the first group (CPG) was supplemented daily for 7 days with 500 mg of curcumin + 20 mg piperine, and the second group (PG) was supplemented with 540 mg of cellulose. After the 7th day of supplementation, the volunteers participated in the experimental running protocol, where blood samples were collected before, after, and one hour after exercise for analysis of the number of leukocytes, creatine kinase, and cytokine concentration (IL-2, TNF-α, IFN, IL-6, and IL-10) using flow cytometry. This process was repeated, reversing the supplementation offered to the groups. Results: curcumin and piperine supplementation could not change the physical performance, immune cell counts, and muscle damage; however, the aerobic fatiguing exercise protocol inhibited the elevation of the plasmatic levels of some cytokines. The running exercise protocol could elevate the circulating levels of IL-2 (from 49.7 to 59.3 pg/mL), TNF-α (from 48.5 to 51.5 pg/mL), INF (from 128.8 to 165.0 pg/mL), IL-6 (from 63.1 to 77.3 pg/mL), and IL-10 (from 48.9 to 59.6 pg/mL) 1 h after the end of the running protocol. However, the curcumin and piperine supplementation could inhibit this elevation. Conclusions: curcumin and piperine supplementation had no effect on physical performance, immune cell counts, or muscle damage; however, the supplementation could modulate the kinetics of IL-2, TNF-α, INF, IL-6, and IL-10 1 h after the end of exercise.
