Phylogeny of Shrew- and Mole-Borne Hantaviruses in Poland and Ukraine
Fuka Kikuchi,
Satoru Arai,
Janusz Hejduk,
Ai Hayashi,
Janusz Markowski,
Marcin Markowski,
Leszek Rychlik,
Vasyl Khodzinskyi,
Hajime Kamiya,
Tetsuya Mizutani,
Motoi Suzuki,
Beata Sikorska,
Paweł P. Liberski,
Richard Yanagihara
Affiliations
Fuka Kikuchi
Center for Surveillance, Immunization and Epidemiologic Research, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Satoru Arai
Center for Surveillance, Immunization and Epidemiologic Research, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Janusz Hejduk
Department of Biodiversity Studies and Bioeducation, Faculty of Biology and Environmental Protection, University of Łódź, 90-237 Łódź, Poland
Ai Hayashi
Center for Surveillance, Immunization and Epidemiologic Research, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Janusz Markowski
Department of Biodiversity Studies and Bioeducation, Faculty of Biology and Environmental Protection, University of Łódź, 90-237 Łódź, Poland
Marcin Markowski
Department of Experimental Zoology and Evolutionary Biology, Faculty of Biology and Environmental Protection, University of Łódź, 90-237 Łódź, Poland
Leszek Rychlik
Department of Systematic Zoology, Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznań, Poland
Vasyl Khodzinskyi
Institute of Forestry and Park Gardening, Ukrainian National Forestry University, 79057 Lviv, Ukraine
Hajime Kamiya
Center for Surveillance, Immunization and Epidemiologic Research, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Tetsuya Mizutani
Center for Infectious Diseases Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
Motoi Suzuki
Center for Surveillance, Immunization and Epidemiologic Research, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
Beata Sikorska
Department of Molecular Pathology and Neuropathology, Medical University of Łódź, 92-216 Łódź, Poland
Paweł P. Liberski
Department of Molecular Pathology and Neuropathology, Medical University of Łódź, 92-216 Łódź, Poland
Richard Yanagihara
Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA
Earlier, we demonstrated the co-circulation of genetically distinct non-rodent-borne hantaviruses, including Boginia virus (BOGV) in the Eurasian water shrew (Neomys fodiens), Seewis virus (SWSV) in the Eurasian common shrew (Sorex araneus) and Nova virus (NVAV) in the European mole (Talpa europaea), in central Poland. To further investigate the phylogeny of hantaviruses harbored by soricid and talpid reservoir hosts, we analyzed RNAlater®-preserved lung tissues from 320 shrews and 26 moles, both captured during 1990–2017 across Poland, and 10 European moles from Ukraine for hantavirus RNA through RT-PCR and DNA sequencing. SWSV and Altai virus (ALTV) were detected in Sorex araneus and Sorex minutus in Boginia and the Białowieża Forest, respectively, and NVAV was detected in Talpa europaea in Huta Dłutowska, Poland, and in Lviv, Ukraine. Phylogenetic analyses using maximum-likelihood and Bayesian methods showed geography-specific lineages of SWSV in Poland and elsewhere in Eurasia and of NVAV in Poland and Ukraine. The ATLV strain in Sorex minutus from the Białowieża Forest on the Polish–Belarusian border was distantly related to the ATLV strain previously reported in Sorex minutus from Chmiel in southeastern Poland. Overall, the gene phylogenies found support long-standing host-specific adaptation.
