Drosophila Model of Leishmania amazonensis Infection

Kendi Okuda; Neal Silverman

doi:10.21769/BioProtoc.2640

Bio-Protocol (Dec 2017)

Drosophila Model of Leishmania amazonensis Infection

Kendi Okuda,
Neal Silverman

Affiliations

Kendi Okuda: Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Neal Silverman: Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA

DOI: https://doi.org/10.21769/BioProtoc.2640
Journal volume & issue: Vol. 7, no. 23

Abstract

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This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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