Nature Communications (Dec 2020)
Pathway engineering in yeast for synthesizing the complex polyketide bikaverin
- Meng Zhao,
- Yu Zhao,
- Mingdong Yao,
- Hala Iqbal,
- Qi Hu,
- Hong Liu,
- Bin Qiao,
- Chun Li,
- Christine A. S. Skovbjerg,
- Jens Christian Nielsen,
- Jens Nielsen,
- Rasmus J. N. Frandsen,
- Yingjin Yuan,
- Jef D. Boeke
Affiliations
- Meng Zhao
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Yu Zhao
- Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, NYU Langone Health
- Mingdong Yao
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Hala Iqbal
- Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, NYU Langone Health
- Qi Hu
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Hong Liu
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Bin Qiao
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Chun Li
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Christine A. S. Skovbjerg
- Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark
- Jens Christian Nielsen
- Department of Biology and Biological Engineering, Chalmers University of Technology
- Jens Nielsen
- Department of Biology and Biological Engineering, Chalmers University of Technology
- Rasmus J. N. Frandsen
- Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark
- Yingjin Yuan
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
- Jef D. Boeke
- Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, NYU Langone Health
- DOI
- https://doi.org/10.1038/s41467-020-19984-3
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
Bikaverin is a fungal-derived tetracyclic polyketide with antibiotic, antifungal and anticancer properties. Here, the authors employ various pathway engineering strategies to achieve high level production of bikaverin in baker’s yeast.
