The effect of adipose-derived stem cell exosomes on the biological activity of dermal papilla cells

Abstract

Objective To investigate the effects of adipose-derived stem cell exsomes (ADSC-Exos) on dermal papilla cells (DPCs) biological activity, and the effect of ADSC-Exos on hair growth. Methods Adipose-derived stem cells (ADSCs) were cultured in primary culture, and their surface immunomarkers and multidirectional differentiation potential were identified. The supernatants of P3 generation cells were collected, Exosomes (Exos) were isolated and extracted by super centrifugation, and their morphology, particle size and surface markers were identified. ADSC-Exos were co-cultured with DPCs. Cell proliferation assay (CCK-8) and Western blot were performed to evaluate the effects of ADSC-Exos on DPCs proliferation and inducibility. Results The primary ADSCs and ADSC-Exos obtained in this study met the identification criteria of mesenchymal stem cells and exosome characteristics. Compared with the control group, CCK-8 test indicated that the cell viability of DPCs in the exosome group was significantly increased (P<0.05). Western blot showed that the relative expression levels of Versican and alkaline phosphatase (ALP) in the exosome group were higher than those in the control group (all P<0.05). Conclusion ADSCS-Exos can promote the proliferation of DPCs and enhance its hair follicle inducibility.

Keywords

• adipose-derived stem cells
• exosomes
• dermal papilla cells
• proliferation
• inducibility