Generation of two iPSC cell lines (SIAISi020-A and SIAISi019-A) from an 82-year-old mild cognitive impairment (MCI) and her unaffected child from Chinese Han population

Yanfei Ding; Haijuan Chen; Jian Zhao; Ying Wang; Yulei Deng

Stem Cell Research (Aug 2022)

Generation of two iPSC cell lines (SIAISi020-A and SIAISi019-A) from an 82-year-old mild cognitive impairment (MCI) and her unaffected child from Chinese Han population

Yanfei Ding,
Haijuan Chen,
Jian Zhao,
Ying Wang,
Yulei Deng

Affiliations

Yanfei Ding: Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Haijuan Chen: Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Jian Zhao: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China
Ying Wang: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China; Corresponding authors at: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China (Y. Wang). Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China (Y. Deng).
Yulei Deng: Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authors at: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China (Y. Wang). Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China (Y. Deng).

Journal volume & issue: Vol. 63
p. 102869

Abstract

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IPSCs have great potential value in cell replacement therapy, pathogenesis research, screening for new drugs, and treatment of clinical disease. An 82-year-old woman with mild cognitive impairment (MCI) and her unaffected child donated their peripheral blood mononuclear cells (PBMC). Their PBMCs were reprogrammed using human OKSM transcription factors (SOX2, OCT3/4, KLF4 and C-MYC) via a non-integrated complementary vector system. In the newly developed hiPSC series SIAISi019-A and SIAISi020-A, immunocytochemistry and the ability to spontaneously differentiate into 3 germ layers in vitro confirmed the pluripotency of transgene-free iPSCs. And their karyotypes were normal.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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