Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
Ma. Florencia Peralta,
Ma. Laura Guzman,
Ma. Estefanía Bracamonte,
J. Diego Marco,
Ma. Eugenia Olivera,
Dolores C. Carrer,
Paola A. Barroso
Affiliations
Ma. Florencia Peralta
Instituto Ferreyra - INIMEC-CONICET – Universidad Nacional de Córdoba, Córdoba, Argentina
Ma. Laura Guzman
Unidad de Investigación y Desarrollo en Tecnología Farmacéutica – CONICET and Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina
Ma. Estefanía Bracamonte
Instituto de Patología Experimental – CONICET and Universidad Nacional de Salta, Salta, Argentina
J. Diego Marco
Instituto de Patología Experimental – CONICET and Universidad Nacional de Salta, Salta, Argentina
Ma. Eugenia Olivera
Unidad de Investigación y Desarrollo en Tecnología Farmacéutica – CONICET and Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina
Dolores C. Carrer
Instituto Ferreyra - INIMEC-CONICET – Universidad Nacional de Córdoba, Córdoba, Argentina; Corresponding author.
Paola A. Barroso
Instituto de Patología Experimental – CONICET and Universidad Nacional de Salta, Salta, Argentina; Corresponding author.
An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work was to investigate the in vivo efficacy of topical Ris and EuE-Ris complexes on CL. Surface charge and Ris release kinetics from the different dispersions were analyzed. BALB/c mice were infected intradermally with promastigotes of L. (L.) amazonensis. Ulcers were treated with Ris or EuE-Ris hydrogels. All the lesions that received topical Ris or EuE-Ris showed an improvement with respect to control: reduction of ulcer average size, cicatrization, flattened edges and no signs of necrosis. In addition, a marked parasitic inhibition of 69.5 and 73.7% was observed in the groups treated with Ris and EuE-Ris, respectively, with the IgG2a levels indicating a tendency towards cure. The results are promising and the system should now be enhanced to achieve total parasite elimination.
