Stem Cell Research (Jul 2018)

Generation of a human induced pluripotent stem cell line from a patient with a rare A673T variant in amyloid precursor protein gene that reduces the risk for Alzheimer's disease

  • Šárka Lehtonen,
  • Ida Höytyläinen,
  • Jenni Voutilainen,
  • Tuuli-Maria Sonninen,
  • Johanna Kuusisto,
  • Markku Laakso,
  • Riikka H. Hämäläinen,
  • Minna Oksanen,
  • Jari Koistinaho

Journal volume & issue
Vol. 30
pp. 96 – 99

Abstract

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An amyloid precursor protein (APP) A673T mutation was found to be protective against Alzheimer's disease (AD) and cognitive decline in the Icelandic population and to associate with decreased levels of plasma β-amyloid in a Finnish population-based cohort. Human fibroblasts from a Finnish male individual carrying the protective mutation were used to generate integration-free induced pluripotent stem cell (iPSCs) line by Sendai virus technology. The iPSC line retained the mutation and expressed pluripotency markers, had a normal karyotype and differentiated into all three germ layers.